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Thus, the regular severe intra-abdominal hypertension-induced lesions were further affected with decompression and an equal time-period of reperfusion to produce the final considerable ischemia/reperfusion lesions and hemorrhage (i.e., intraventricular hemorrhage involving the third ventricle occurred at the end in rats with grade IV 40 mmHg intra-abdominal hypertension ischemia/reperfusion)

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Importantly, these clones showed no mutations in five of the most likely predicted off-target sites (Supplementary Fig