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For example, biochemical assays for desensitization, such as inhibition of adenylyl cyclase that requires more than 5 minutes of sustained opioid exposure (most assays take 10–20 minutes, e.g., Law et al., 2000, or longer, e.g., Koch et al., 2005), measure the combined effects of rapid desensitization at the cell surface plus endocytosis or recovery from desensitization