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Notably, the inflammatory response was size-dependent, with 75 nm and 110 nm AgNPs inducing more pronounced cellular infiltration than 10 nm particles

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In contrast to histidine binding by OvsA, hercynine binding in SenA is primarily accomplished through electrostatic interactions with the substrate’s N α-methyl groups, including dipolar contacts with the Asn395 side chain and cation-π interactions with Phe396 (Fig